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Phospho-RPS6 (S235/S236) + Total RPS6 TR-FRET Cell Signaling Assay Kit

Phospho-RPS6 (S235/S236) + Total RPS6 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-RPS6APT Reactivity: Human Conjugation: Brand: Bioauxilium
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$1,375 USD
$1,375 USD
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Product Details

Product Specification


Antigen Ribosomal Protein S6
Reactivity Human
Components

400 points for phospho; 100 points for total


Eu-phospho-RPS6S235
Acc-phospho-RPS6S235
Eu-Total RPS6
Acc-Total RPS6
RPS6 control lysate
5X Lysis Buffer 4
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Ribosomal Protein S6 (S235/S236) and Total Ribosomal Protein S6 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total Ribosomal Protein S6 proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with insulin stimulates phosphorylation of RPS6 at S235/S236, but does not affect the levels of total RPS6
Inhibition of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of RPS6 at S235/S236 by insulin, but does not affect the levels of total RPS6
MCF7 control lysate titration (QC Test) Phospho-RPS6 (S235/S236)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
MCF7 control lysate titration (QC Test) total RPS6
Data show a linear relationship between lysate dilutions and TR-FRET ratio values