WB result of Phospho-RIP3 (Thr231/Ser232) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-RIP3 (Thr231/Ser232) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated L-929 whole cell lysate 20 µg
Lane 2: L-929 treated with 20 μM Z-VAD for 30 minutes, then treated with 20 ng/ml mTNF-α and 100 nM SM-164 for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 54 kDa
This blot was developed with high sensitivity substrate
Phospho-RIP3 (Thr231/Ser232) Recombinant Rabbit mAb (S-3487)
Phospho-RIP3 (Thr231/Ser232) Recombinant Rabbit mAb (S-3487)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-RIP3 (Thr231/Ser232) |
| Synonyms | Receptor-interacting serine/threonine-protein kinase 3; RIP-like protein kinase 3; Receptor-interacting protein 3 (RIP-3; mRIP3); Rip3; Ripk3 |
| Location | Cytoplasm, Nucleus |
| Accession | Q9QZL0 |
| Clone Number | S-3487 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Ms |
Background
Phospho-RIP3 (Thr231/Ser232) is the phosphorylated, catalytically active form of the 57 kDa serine/threonine kinase Receptor-Interacting Protein 3 (RIP3, RIPK3); dual phosphorylation at Thr231 and Ser232 within the conserved activation loop is induced by TNF-α, TRAIL or viral infection, converting RIP3 into a core driver of necroptosis—a programmed, caspase-independent inflammatory cell death pathway—via autophosphorylation and subsequent phosphorylation of downstream substrates such as MLKL, thereby assembling the necrosome complex and triggering membrane rupture, calcium influx and damage-associated molecular pattern (DAMP) release; detection of Phospho-RIP3 (Thr231/Ser232) by phospho-specific antibodies is therefore a key biomarker for necroptotic signaling in cancer, ischemia-reperfusion injury and inflammatory diseases.
Picture
Picture
Western Blot
