WB result of Phospho-RIP3 (Ser232) Recombinant Rabbit mAb
Primary antibody: Phospho-RIP3 (Ser232) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated L-929 whole cell lysate 20 µg
Lane 2: L-929 treated with 20 μM Z-VAD for 30 minutes, then treated with 100 nM SM-164 and 20 ng/ml TNF-α for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 54 kDa
Phospho-RIP3 (Ser232) Recombinant Rabbit mAb (S-1134-6)
Phospho-RIP3 (Ser232) Recombinant Rabbit mAb (S-1134-6)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-RIP3 (Ser232) |
| Synonyms | Receptor-interacting serine/threonine-protein kinase 3; RIP-like protein kinase 3; Receptor-interacting protein 3 (RIP-3); RIP3; RIPK3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q9Y572 |
| Clone Number | S-1134-6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Ms |
Background
Phospho-RIP3 (Ser232) is a phosphorylated form of the receptor-interacting serine/threonine-protein kinase 3 (RIPK3), which plays a crucial role in regulating necroptosis, a form of programmed cell death. When phosphorylated at Ser232, RIPK3 is activated and forms a necrosis-inducing complex with other proteins such as RIPK1 and MLKL. This phosphorylation event is essential for the recruitment and activation of MLKL, which subsequently executes necroptosis by causing plasma membrane damage and calcium influx. Additionally, Phospho-RIP3 (Ser232) can also participate in apoptosis regulation and metabolic processes in certain cell types.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-RIP3 (Ser232) Recombinant Rabbit mAb
Lane 1: RIP3 (Ser232) phospho peptide
Lane 2: RIP3 unmodified peptide
Primary antibody: Phospho-RIP3 (Ser232) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
