WB result of Phospho-Rb (Ser780) Recombinant Mouse mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Rb (Ser780) Recombinant Mouse mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 110 kDa
Phospho-Rb (Ser780) Recombinant Mouse mAb (S-3521)
Phospho-Rb (Ser780) Recombinant Mouse mAb (S-3521)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Phospho-Rb (Ser780) |
| Synonyms | Retinoblastoma-associated protein; p105-Rb; p110-RB1; pRb (Rb); pp110; RB1 |
| Location | Cytoplasm, Nucleus |
| Accession | P06400 |
| Clone Number | S-3521 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB |
| Reactivity | Hu, Mk |
| Positive Sample | Jurkat, Molt-4, COS-7 |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
Background
Phospho-Rb (Ser780) refers to the specific modification form of the retinoblastoma protein (Rb) phosphorylated at its serine 780 residue. The Rb protein is a key regulator of the cell cycle G1/S checkpoint. In its unphosphorylated state, it halts cell cycle progression by binding to and inhibiting E2F transcription factors. When cells receive proliferative signals, the cyclin-dependent kinase 4/6-cyclin D complex specifically catalyzes the phosphorylation of the Rb protein at the Ser780 site. This event is an early and critical step in the inactivation process of the Rb protein. Phosphorylation at Ser780 directly disrupts the interaction between Rb and E2F, thereby relieving the inhibition. The released E2F then activates the transcription of a suite of genes necessary for S-phase entry, driving the cell cycle forward. Consequently, detecting the level of Phospho-Rb (Ser780) is commonly used to assess CDK4/6 activity and the state of cell proliferation, holding significant importance in cancer research and the development of targeted therapies, such as CDK4/6 inhibitors.
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Picture
Western Blot
WB result of Phospho-Rb (Ser780) Recombinant Mouse mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Rb (Ser780) Recombinant Mouse mAb at 1/1000 dilution
Lane 1: Molt-4 treated with 500 ng/ml Ionomycin and 10 ng/m PMA for 24 hours whole cell lysate 20 µg
Lane 2: Molt-4 treated with 500 ng/ml Ionomycin and 10 ng/m PMA for 24 hours whole cell lysate 20 µg (phosphatase treated membrane)
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 110 kDa
WB result of Phospho-Rb (Ser780) Recombinant Mouse mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Rb (Ser780) Recombinant Mouse mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 110 kDa
