WB result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDa
Phospho-PKA C (Thr197) Recombinant Rabbit mAb (S-1137-310)
Phospho-PKA C (Thr197) Recombinant Rabbit mAb (S-1137-310)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-PKA C (Thr197) |
| Synonyms | cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PKACA; PRKACA |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | P17612 |
| Clone Number | S-1137-310 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Predicted Reactivity | Bv, C.el, Dg, Dr, Hm, Pg, Sh |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| ICC | 1:500 | Hu |
Background
Phospho-PKA C (Thr197) is the catalytic subunit of cAMP-dependent protein kinase A that carries a single phosphate group on threonine 197 within its activation loop; this modification, catalyzed by the upstream kinase PDK1, locks the kinase in an active conformation, enhances substrate turnover, and serves as a biomarker of PKA pathway activation in response to hormones such as epinephrine and glucagon, making it a critical node in signal transduction, synaptic plasticity, and metabolic regulation.
Picture
Picture
Western Blot
WB result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: phosphatase treated NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDa
WB result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: phosphatase treated C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDa
WB result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDa
Dot Blot
Dot blot result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Lane 1: PKA C (Thr197) phospho peptide
Lane 2: PKA C (Thr197) unmodified peptide
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of HeLa cells starved overnight, then treated with Calyculin A (100nM, 30 min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-PKA C (Thr197) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
