WB result of Phospho-p90RSK (Ser380) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-p90RSK (Ser380) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starved overnight, then treated with 200 nM TPA for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 90 kDa
Phospho-p90RSK (Ser380) Recombinant Rabbit mAb (S-4985)
Phospho-p90RSK (Ser380) Recombinant Rabbit mAb (S-4985)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-p90RSK (Ser380) |
| Synonyms | Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1; p90RSK1; p90S6K); MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a); Ribosomal S6 kinase 1 (RSK-1); RPS6KA1; MAPKAPK1A; RSK1; Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 2 (p90-RSK 2; p90RSK2); MAP kinase-activated protein kinase 1c (MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c); Ribosomal S6 kinase 3 (RSK-3); pp90RSK3; RPS6KA2; MAPKAPK1C; RSK3; Ribosomal protein S6 kinase alpha-3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 3 (p90-RSK 3; p90RSK3); Insulin-stimulated protein kinase 1 (ISPK-1); MAP kinase-activated protein kinase 1b (MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b); Ribosomal S6 kinase 2 (RSK-2); pp90RSK2; RPS6KA3; ISPK1; MAPKAPK1B; RSK2 |
| Location | Cytoplasm, Nucleus |
| Accession | P51812、Q15349、Q15418 |
| Clone Number | S-4985 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Mk |
| Positive Sample | HeLa (starved overnight, then treated with 200 nM TPA for 10 minutes), COS-7 (treated with 200 nM TPA for 30 minutes) |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
Background
Phospho-p90RSK (Ser380) refers to the p90 ribosomal S6 kinase (p90RSK) protein specifically phosphorylated at serine residue 380, a critical post-translational modification that serves as a key indicator of the kinase's activation status within the Ras/MAPK signaling pathway. As a downstream effector of ERK1/2, p90RSK undergoes sequential phosphorylation events where phosphorylation at Ser380 in the C-terminal kinase domain facilitates the subsequent phosphorylation of the N-terminal kinase domain, thereby fully activating the enzyme to regulate diverse cellular processes including cell proliferation, survival, differentiation, and motility. This specific phospho-epitope is widely utilized in biomedical research as a reliable biomarker for monitoring MAPK pathway activity and assessing the efficacy of targeted therapies in various pathological conditions, particularly in cancers where aberrant RSK signaling contributes to tumor growth and resistance to apoptosis.
Picture
Picture
Western Blot
WB result of Phospho-p90RSK (Ser380) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-p90RSK (Ser380) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated COS-7 whole cell lysate 20 µg
Lane 2: COS-7 treated with 200 nM TPA for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 90 kDa
