WB result of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb
Primary antibody: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 200 nM TPA for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42, 44 kDa
Observed MW: 38, 40 kDa
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb (S-602-276)
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb (S-602-276)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P27361、 P28482 |
| Clone Number | S-602-276 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | C.el, Dr, Xe, Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| IP | 1:50 |
Background
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) refers to the phosphorylated form of the mitogen-activated protein kinases, also known as extracellular signal-regulated kinases 1 and 2. These kinases are part of the MAPK signaling pathway, which is a series of protein kinases that play a crucial role in regulating various cellular processes such as cell growth, differentiation, proliferation, and apoptosis. The phosphorylation of Erk1/2 at specific residues, Thr202 and Tyr204, is a key regulatory event that activates these kinases. When Erk1/2 are phosphorylated at these sites, they become active and can then phosphorylate a variety of target proteins, thereby transmitting signals from the cell membrane to the nucleus.
Picture
Picture
Western Blot
WB result of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb
Primary antibody: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 200 nM TPA for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42, 44 kDa
Observed MW: 38, 40 kDa
WB result of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb
Primary antibody: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 200 nM TPA for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42, 44 kDa
Observed MW: 38, 40 kDa
IP
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) in 0.4 mg Jurkat treated with 200 nM TPA for 20 minutes whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Jurkat treated with 200 nM TPA for 20 minutes whole cell lysate 20 µg (Input)
Lane 2: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Rabbit mAb IP in Jurkat treated with 200 nM TPA for 20 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Jurkat treated with 200 nM TPA for 20 minutes whole cell lysate
Predicted MW: 42, 44 kDa
Observed MW: 42, 44 kDa
Dot Blot
Dot blot result of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb
Lane 1: p44/42 MAPK (Erk1/2) (Thr202/Tyr204) phospho peptide
Lane 2: p44/42 MAPK (Erk1/2) (Thr202) phospho peptide
Lane 3: p44/42 MAPK (Erk1/2) (Thr204) phospho peptide
Lane 4: p44/42 MAPK (Erk1/2) unmodified peptide
Primary antibody: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
