WB result of Phospho-NRF2 (Ser40) Recombinant Rabbit mAb
Primary antibody: Phospho-NRF2 (Ser40) Recombinant Rabbit mAb at 1/250 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: phosphatase treated HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 110 kDa
Phospho-NRF2 (Ser40) Recombinant Rabbit mAb (S-1720-97)
Phospho-NRF2 (Ser40) Recombinant Rabbit mAb (S-1720-97)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-NRF2 (Ser40) |
| Synonyms | Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; Nrf-2; Nuclear factor; erythroid derived 2, like 2; NFE2L2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q16236 |
| Clone Number | S-1720-97 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:250 | Hu |
| IHC-P | 1:500 | Hu |
Background
Phospho-NRF2 (Ser40) is a crucial form of the NRF2 protein, which is a basic leucine zipper transcription factor involved in regulating the antioxidant response. When phosphorylated at Ser40 by protein kinase C (PKC), NRF2 dissociates from its repressor KEAP1, leading to its nuclear translocation and activation. This activation results in the upregulation of antioxidant genes containing antioxidant response elements (AREs), helping cells combat oxidative stress. The phosphorylation of NRF2 at Ser40 is essential for its stability and function, and it plays a significant role in various cellular processes, including protection against oxidative damage and regulation of metabolic pathways.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-NRF2 (Ser40) Recombinant Rabbit mAb
Lane 1: NRF2 (Ser40) phospho peptide 1
Lane 2: NRF2 (Ser40) phospho peptide 2
Lane 3: NRF2 (Ser40) unmodified peptide
Primary antibody: Phospho-NRF2 (Ser40) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon (Left) and negative staining in human colon treated with phosphatase (Right). Anti- Phospho-NRF2 (Ser40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma (Left) and negative staining in human lung adenocarcinoma treated with phosphatase (Right). Anti- Phospho-NRF2 (Ser40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
