WB result of Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
Lane 3: NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes, then treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 105 kDa
Observed MW: 110 kDa
Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb (S-1146-117)
Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb (S-1146-117)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-NF-κB p105 (Ser932) |
| Synonyms | Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P19838 |
| Clone Number | S-1146-117 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms, Rt |
| Predicted Reactivity | Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
Background
Phospho-NF-κB p105 (Ser932) refers to the phosphorylated form of the NF-κB p105 protein at the serine residue 932. This post-translational modification is significant as it triggers a series of events that lead to the activation of the NF-κB signaling pathway, which is crucial in regulating immune and inflammatory responses. When p105 is phosphorylated at Ser932 by the IKK complex, it recruits the SCFβTrCP E3 ligase, leading to the ubiquitination and subsequent proteasomal degradation of p105. This degradation releases the active form of TPL-2, which in turn activates the MEK/ERK signaling pathway. Therefore, phosphorylation at Ser932 is a key regulatory step in controlling NF-κB activation and MAP kinase signaling, which coordinate the functions of lymphocytes and macrophages in the immune response.
Picture
Picture
Western Blot
WB result of Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 105 kDa
Observed MW: 110 kDa
