Phospho-Lyn (Tyr396) Recombinant Rabbit mAb

Phospho-Lyn (Tyr396) refers to the active form of the non-receptor tyrosine kinase Lyn (a Src family member) generated by autophosphorylation at Tyr396 within its activation loop. Full activation of Lyn typically requires two steps: first, phosphatases (such as Shp2) remove phosphorylation at the C-terminal inhibitory site Tyr507, and subsequently Lyn undergoes autophosphorylation at Tyr396, a modification that alters the conformation of the activation loop and makes the ATP-binding pocket more accessible, thereby enhancing kinase activity. In the B-cell receptor (BCR) signaling pathway, Lyn Tyr396 phosphorylation is a key marker of early signaling events following BCR activation and drives activation of downstream pathways including BTK, ERK, and NFκB. In a subset of patients with chronic lymphocytic leukemia (CLL), Lyn is constitutively active at Tyr396 and further phosphorylates HS1 at Tyr397, promoting cytoskeletal remodeling, migration, and adhesion; inhibition of Lyn with dasatinib attenuates this signaling axis and reduces leukemia cell survival. Notably, in experimental studies it is essential to strictly distinguish the active form of Lyn (pY396) from the inhibitory form (pY507); some early literature misread pY507 as an activation marker owing to antibody specificity issues.