WB result of Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb
Primary antibody: Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with a construct expressing myc-His-tagged full-length LRP6 fusion protein whole cell lysate 20 µg
Lane 3: 293T transfected with a construct expressing myc-His-tagged full-length LRP6 fusion protein, then treated with 100 ng/ml Wnt-3a for 15 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 180 kDa
Observed MW: 200 kDa
Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb (S-1489-86)
Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb (S-1489-86)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-LRP6 (Ser1490) |
| Synonyms | Low-density lipoprotein receptor-related protein 6; LRP-6 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | O75581 |
| Clone Number | S-1489-86 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Background
Phospho-LRP6 (Ser1490) is a key phosphorylation site on the LRP6 protein, which is a component of the Wnt-Fzd-LRP5-LRP6 complex that initiates the β-catenin signaling pathway. This phosphorylation event is crucial for the aggregation of receptor-ligand complexes into ribosome-sized signalsomes, which are essential for the activation of the Wnt/β-catenin signaling. The phosphorylation of LRP6 at Ser1490, mediated by kinases such as GSK3 and CK1, is required for the binding of AXIN1 and subsequent stabilization and activation of β-catenin by preventing GSK3-mediated phosphorylation of β-catenin. This process is pivotal in various biological processes, including bone formation and embryonic development. The Phospho-LRP6 (Ser1490) antibody specifically detects endogenous levels of LRP6 only when it is phosphorylated at Ser1490, indicating the importance of this modification in the regulation of Wnt signaling.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb
Lane 1: LRP6 (Ser1490) phospho peptide
Lane 2: LRP6 (Ser1490) phospho peptide
Lane 3: LRP6 WT peptide
Primary antibody: Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
