WB result of Phospho-LKB1 (Ser428) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-LKB1 (Ser428) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated COS-7 whole cell lysate 20 µg
Lane 2: COS-7 treated with 200 nM TPA for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 55 kDa
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-LKB1 (Ser428) |
| Synonyms | Serine/threonine-protein kinase STK11; Liver kinase B1 (LKB1; hLKB1); Renal carcinoma antigen NY-REN-19; LKB1; PJS; STK11 |
| Location | Cytoplasm, Nucleus, Mitochondrion |
| Accession | Q15831 |
| Clone Number | S-3489 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Picture
Picture
Western Blot
