WB result of Phospho-Lck (Tyr394) Recombinant Rabbit mAb
Primary antibody: Phospho-Lck (Tyr394) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 5 µg
Lane 2: Jurkat treated with 50mM pervanadate for 5 minutes whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 55-70 kDa
Phospho-Lck (Tyr394) Recombinant Rabbit mAb (S-1957-74)
Phospho-Lck (Tyr394) Recombinant Rabbit mAb (S-1957-74)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Lck (Tyr394) |
| Synonyms | Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase (LSK); Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK; LCK |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | P06239 |
| Clone Number | S-1957-74 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv, Ck, CyMk, Dg, Pg, Xe, Zf |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000-1:5000 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
Phospho-Lck (Tyr394) protein is a phosphorylated form of the Lck protein at tyrosine residue 394. Lck, or lymphocyte-specific protein tyrosine kinase, is a non-receptor tyrosine kinase that plays a crucial role in the early stages of T-cell activation. When phosphorylated at Tyr394, Lck becomes fully activated, enabling it to initiate downstream signaling cascades. This phosphorylation event is essential for the proper functioning of the immune system, as it helps in the transmission of signals from the T-cell receptor to intracellular effectors, ultimately leading to T-cell proliferation and differentiation. Abnormal regulation of Phospho-Lck (Tyr394) has been implicated in various immune disorders, making it an important target for therapeutic interventions.
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Picture
Western Blot
WB result of Phospho-Lck (Tyr394) Recombinant Rabbit mAb
Primary antibody: Phospho-Lck (Tyr394) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 55 kDa
Dot Blot
Dot blot result of Phospho-Lck (Tyr394) Recombinant Rabbit mAb
Lane 1: Lck (Tyr394) phospho peptide 1
Lane 2: Lck (Tyr394) phospho peptide 2
Lane 3: Lck (Tyr394) unmodified peptide
Primary antibody: Phospho-Lck (Tyr394) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of Jurkat cells treated with 50mM pervanadate for 5 minutes (top panel) and untreated Jurkat cells (below panel). Anti- Phospho-Lck (Y394) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
