WB result of Phospho-IKKα/β (Ser176/180) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-IKKα/β (Ser176/180) Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 20 ng/ml TNF-alpha and 50 nM Calyculin A for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
Phospho-IKKα/β (Ser176/180) Recombinant Rabbit mAb (S-4059)
Phospho-IKKα/β (Ser176/180) Recombinant Rabbit mAb (S-4059)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-IKKα/β (Ser176/180) |
| Synonyms | Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1 (IKK-1; IKK1); Nuclear factor NF-kappa-B inhibitor kinase alpha (NFKBIKA); Transcription factor 16 (TCF-16); IKKA; TCF16; CHUK |
| Location | Cytoplasm, Nucleus |
| Accession | O15111 |
| Clone Number | S-4059 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | TNF-alpha and Calyculin A treated HeLa |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
Phospho-IKKα/β (Ser176/180) refers to the phosphorylated, catalytically active forms of the IκB kinase (IKK) catalytic subunits IKKα (at Ser176/Ser180) and IKKβ (at Ser177/Ser181); these activating phosphorylation events, induced by inflammatory cytokines such as TNF-α and IL-1β, trigger a conformational switch that fully activates the IKK complex, enabling it to phosphorylate the NF-κB inhibitor IκBα and thus liberate the transcription factor NF-κB for nuclear import and pro-inflammatory gene expression.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Phospho-IKKα/β (Ser176/180) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Phospho-IKKα/β (Ser176/180) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- Phospho-IKKα/β (Ser176/180) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- Phospho-IKKα/β (Ser176/180) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
