WB result of Phospho-Histone H3 (Ser10) Rabbit pAb
Primary antibody: Phospho-Histone H3 (Ser10) Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Nocodazole for 18 hours, then treated with 100 nM Calyculin A for 1 hr whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody
Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody
Price:
Regular price
$100.00 USD
Regular price
Sale price
$100.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Histone H3 (Ser10) |
| Synonyms | H3S10P |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Isotype | IgG |
| Application | WB, ChIP |
| Reactivity | Hu |
| Predicted Reactivity | Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe |
| Purification | Immunogen Affinity |
| Concentration | 4 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| ChIP | 1:20-1:50 |
Background
H3S10P is an epigenetic modification to the DNA packaging protein histone H3. It is a mark that indicates the phosphorylation the 10th serine residue of the histone H3 protein. The S10 phosphorylation is involved in mitosis, transcription, chromatin condensation, and UVB response. H3S10p causes chromosome condensation and segregation during cell mitosis. H3S10p temporarily increases during mitosis while H3K9me3 decareses and H3K9me3 recovers upon mitotic exit.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-Histone H3 (Ser10) Rabbit pAb
Lane1: Histone H3 (Ser10) phospho peptide
Lane2: Histone H3 (Ser10) phospho peptide
Lane3: Histone H3 non-phospho peptide
Primary antibody: Phospho-Histone H3 (Ser10) Rabbit pAb at 1/1000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa + Nocodazole (100ng/ml,18h) (+) (upper panel) and HeLa + Nocodazole (100ng/ml,18h) (-) (lower panel) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented bysonication.
Parallel reactions used Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA)
showed the enrichment of RPL30 and GAPDH in Phospho-Histone H3
(Ser10) Rabbit Polyclonal Antibody -immunoprecipitated sample.
