WB result of Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb
Primary antibody: Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb (S-1368-15)
Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb (S-1368-15)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Histone H2A.X (Ser139) |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P16104 |
| Clone Number | S-1368-15 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC-P | 1:200-1:500 |
Background
Phospho-Histone H2A.X (Ser139), also known as γH2A.X, is a variant of the histone H2A protein that plays a crucial role in the cellular response to DNA double-strand breaks (DSBs). When DNA damage occurs, such as that induced by ionizing radiation or anticancer drugs, the protein is rapidly phosphorylated at Ser139 by kinases like ATM, ATR, and DNA-PK. This phosphorylation event is one of the earliest responses to DNA damage and is essential for the recruitment of various DNA repair proteins to the site of the break.
Picture
Picture
Western Blot
IP
Phospho-Histone H2A.X (Ser139) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Histone H2A.X (Ser139) in 0.4 mg HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-Histone H2A.X (Ser139) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate 20 µg (Input)
Lane 2: Phospho-Histone H2A.X (Ser139) Rabbit mAb IP in HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate
Predicted MW: 15 kDa
Observed MW: 17 kDa
Dot Blot
Dot blot result of Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb
Lane 1: Histone H2A.X (Ser139) phospho peptide
Lane 2: Histone H2A.X unmodified peptide
Primary antibody: Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
