WB result of Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb
Primary antibody: Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: phosphatase treated HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47, 51 kDa
Observed MW: 47, 51 kDa
Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb (S-1810-101)
Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb (S-1810-101)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho - GSK-3α/β (Tyr216/279) |
| Synonyms | Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P49841、 P49840 |
| Clone Number | S-1810-101 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
Background
Phospho - GSK-3α/β (Tyr216/279) proteins are key players in cellular signaling pathways. Glycogen synthase kinase-3 (GSK-3) exists in two isoforms, α and β, and phosphorylation at specific tyrosine residues, such as Tyr216 in GSK-3β and Tyr279 in GSK-3α, can significantly impact their activity. This phosphorylation typically enhances the kinase activity of GSK-3, allowing it to regulate a wide range of biological processes including glycogen metabolism, gene transcription, and cell fate determination. Dysregulation of GSK-3 phosphorylation has been implicated in various diseases, making it an important target for therapeutic interventions.
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Picture
Western Blot
WB result of Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb
Primary antibody: Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: phosphatase treated mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47, 51 kDa
Observed MW: 47, 51 kDa
WB result of Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb
Primary antibody: Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: phosphatase treated rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47, 51 kDa
Observed MW: 47, 51 kDa
Dot Blot
Dot blot result of Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb
Lane 1: GSK-3α/β (Tyr216/279) phospho peptide
Lane 2: GSK-3α/β (Tyr216/279) unmodified peptide
Primary antibody: Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
