WB result of Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starved overnight, then treated with 50 ng/ml hEGF for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 190 kDa
Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb (S-3538-42)
Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb (S-3538-42)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Erk5 (Thr218/Tyr220) |
| Synonyms | Mitogen-activated protein kinase 7; MAP kinase 7; MAPK 7; Big MAP kinase 1 (BMK-1); Extracellular signal-regulated kinase 5 (ERK-5); BMK1; ERK5; PRKM7; MAPK7 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q13164 |
| Clone Number | S-3538-42 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa (starved overnight, then treated with 50 ng/ml hEGF for 10 minutes), NIH/3T3 (starved overnight, then treated with 100 ng/ml PDGF for 5 minutes) |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
Phospho-Erk5 (Thr218/Tyr220) is a phosphorylated form of the extracellular signal-regulated kinase 5 (Erk5), also known as big MAP kinase 1 (BMK1). It is activated through dual phosphorylation at threonine 218 and tyrosine 220 within its TEY activation motif by upstream MAP kinase kinases MEK5. This phosphorylation event is essential for Erk5 catalytic activity and its subsequent nuclear translocation, where it regulates gene expression involved in cell proliferation, survival, and differentiation. Phospho-Erk5 serves as a specific biomarker for Erk5 pathway activation in various cellular processes and disease states, including cancer progression and cardiovascular development.
Picture
Picture
Western Blot
WB result of Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 starved overnight, then treated with 100 ng/ml PDGF for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 190 kDa
