WB result of Phospho-EGF Receptor (Tyr1068) Rabbit pAb
Primary antibody: Phospho-EGF Receptor (Tyr1068) Rabbit pAb at 1/1000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 strave overnight, then treated with 100 ng/ml hEGF for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 180 kDa
Phospho-EGFR (Tyr1068) Rabbit Polyclonal Antibody
Phospho-EGFR (Tyr1068) Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-EGFR (Tyr1068) |
| Synonyms | Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Secreted, Nucleus, Endosome, Endoplasmic reticulum |
| Accession | P00533 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | A431 |
| Predicted Reactivity | Mq |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
Phospho-EGFR (Tyr1173) is a phosphorylated form of the epidermal growth factor receptor (EGFR), a 170 kDa transmembrane receptor tyrosine kinase that plays a crucial role in cell signaling. When EGFR binds to its ligands, such as EGF, it undergoes dimerization and autophosphorylation, activating multiple downstream signaling pathways. Phosphorylation at Tyr1173 is particularly important as it provides a docking site for proteins like the tyrosine phosphatase SHP-1, which can modulate EGFR activity and downstream signaling. This site is also involved in the MAP kinase signaling pathway, which regulates cell growth, survival, and transformation. Abnormal regulation of EGFR, including phosphorylation at Tyr1173, is often associated with various cancers, making it a key target for therapeutic interventions.
Picture
Picture
Western Blot
Immunocytochemistry
ICC analysis of A431 cells treated with EGF (200 ng/mL for 10 min) (top panel) and untreated A431 cells (below panel). Anti- Phospho-EGF Receptor (Tyr1068) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
