WB result of Phospho-DRP1 (Ser616) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-DRP1 (Ser616) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with 100 ng/ml Nocodazole for 17 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate
Phospho-DRP1 (Ser616) Recombinant Rabbit mAb (S-4221)
Phospho-DRP1 (Ser616) Recombinant Rabbit mAb (S-4221)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-DRP1 (Ser616) |
| Synonyms | Density-regulated protein; Protein DRP1; Smooth muscle cell-associated protein 3 (SMAP-3); DRP1; DENR |
| Location | Cytoplasm |
| Accession | O43583 |
| Clone Number | S-4221 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa (treated with 100 ng/ml Calyculin A for 30 minutes), HeLa (treated with 100 ng/ml Nocodazole for 17 hours), NIH/3T3 (treated with 100 ng/ml Calyculin A for 30 minutes) |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
Phospho-DRP1 (Ser616) denotes the phosphorylation of Dynamin-related protein 1 (DRP1) at serine residue 616, a critical post-translational modification that promotes mitochondrial fission. This specific phosphorylation event is primarily mediated by kinases such as Cyclin-dependent kinase 1 (CDK1/Cyclin B) during mitosis, as well as by extracellular signal-regulated kinase 1/2 (ERK1/2) and Protein Kinase C delta (PKCδ) in response to various cellular stresses or growth signals. Phosphorylation at Ser616 enhances the GTPase activity of DRP1 and facilitates its translocation from the cytosol to the outer mitochondrial membrane, where it oligomerizes to constrict and divide mitochondria. This process is essential for maintaining mitochondrial dynamics, ensuring equal distribution of mitochondria during cell division, and regulating apoptosis; conversely, dysregulation of this site is often associated with pathological conditions involving excessive mitochondrial fragmentation, such as neurodegenerative diseases and cardiac injury.
Picture
Picture
Western Blot
WB result of Phospho-DRP1 (Ser616) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-DRP1 (Ser616) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 80 kDa
