WB result of Phospho-CSF-1R/M-CSF-R (Tyr699) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-CSF-1R/M-CSF-R (Tyr699) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 unboiled whole cell lysate 20 µg
Lane 2: THP-1 starve overnight, then treated with 100 ng/ml hM-CSF for 5 minutes unboiled whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
Phospho-CSF-1R/M-CSF-R (Tyr699) Recombinant Rabbit mAb (S-3553)
Phospho-CSF-1R/M-CSF-R (Tyr699) Recombinant Rabbit mAb (S-3553)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-CSF-1R/M-CSF-R (Tyr699) |
| Synonyms | Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor (CSF-1-R; CSF-1R; M-CSF-R); Proto-oncogene c-Fms; CD115; FMS; CSF1R |
| Location | Cell membrane |
| Accession | P07333 |
| Clone Number | S-3553 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | THP-1 starve overnight, then treated with 100 ng/ml hM-CSF for 5 minutes |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Phospho-CSF-1R/M-CSF-R (Tyr699) specifically refers to the colony-stimulating factor 1 receptor (M-CSF-R, encoded by the proto-oncogene c-fms) phosphorylated at tyrosine residue 699. Located within the kinase insert domain of the receptor, Tyr699 undergoes autophosphorylation upon ligand-induced dimerization (e.g., with M-CSF or IL-34). This phosphorylation event creates a critical docking site for the adaptor protein Grb2, thereby initiating downstream signaling pathways including PI3K/Akt and ERK, which are essential for regulating the survival, proliferation, and differentiation of mononuclear phagocytes such as macrophages and microglia. Consequently, detecting the phosphorylation status of Tyr699 serves as a key indicator of CSF-1R activation and holds significant research value in areas such as cancer (e.g., ovarian and breast cancer) and neurological disorders (e.g., adult-onset leukoencephalopathy).
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Western Blot
