WB result of Phospho-Chk2 (Thr68) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Chk2 (Thr68) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 1 μM camptothecin for 1 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 65 kDa
Phospho-Chk2 (Thr68) Recombinant Rabbit mAb (S-2214-11)
Phospho-Chk2 (Thr68) Recombinant Rabbit mAb (S-2214-11)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Chk2 (Thr68) |
| Synonyms | Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog (Hucds1; hCds1); Checkpoint kinase 2; CDS1; CHK2; RAD53; CHEK2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | O96017 |
| Clone Number | S-2214-11 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | Camptothecin treated HeLa cells |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Background
Phospho-Chk2 (Thr68) is the phosphorylated, active form of checkpoint kinase 2 (Chk2), a serine/threonine protein kinase that acts as a key tumor suppressor and DNA-damage transducer; in response to double-strand breaks the PI3K-like kinase ATM rapidly phosphorylates Chk2 on threonine 68, inducing transient dimerization via phospho-Thr68–FHA domain interactions that license subsequent autophosphorylation in the activation loop, thereby generating fully active Chk2 monomers that enforce cell-cycle arrest by phosphorylating substrates such as p53, BRCA1 and the Cdc25 phosphatases, and whose pathway disruption is frequently observed in cancer.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-Chk2 (Thr68) Recombinant Rabbit mAb
Lane 1: ChK2 (Thr68) phospho peptide
Lane 2: ChK2 unmodified peptide
Primary antibody: Phospho-Chk2 (Thr68) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
