WB result of Phospho-Cdc25A (Ser124) Recombinant Rabbit mAb
Primary antibody: Phospho-Cdc25A (Ser124) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg (phosphatase-treated membrane)
Lane 4: Jurkat whole cell lysate 20 µg (phosphatase-treated membrane)
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 70 kDa
Phospho-Cdc25A (Ser124) Recombinant Rabbit mAb (S-5057-5)
Phospho-Cdc25A (Ser124) Recombinant Rabbit mAb (S-5057-5)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Cdc25A (Ser124) |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P30304 |
| Clone Number | S-5057-5 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, NIH/3T3, PC-12 |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:400 | Hu |
Background
Phospho-Cdc25A (Ser124) refers to the modified form of the cell cycle regulatory protein CDC25A following phosphorylation at serine residue 124. CDC25A is a dual-specificity protein phosphatase that primarily drives cells into S phase and promotes DNA replication during the G1/S transition by removing inhibitory phosphate groups from cyclin-dependent kinases such as CDK2. The Ser124 site can be phosphorylated by the checkpoint kinases CHK1 and CHK2 within DNA damage and stress response pathways, as well as by p38 MAPK under conditions such as osmotic stress. Phosphorylation at this site is generally associated with the ubiquitination and proteasomal degradation of CDC25A, leading to reduced protein levels, suppression of CDK2 activity, and cell cycle checkpoint arrest at the G1/S or S phase, thereby preventing damaged cells from continuing to divide. Consequently, Phospho-Cdc25A (Ser124) is commonly used as a marker for stress-induced phosphorylation of CDC25A and checkpoint activation, and is employed in Western blotting, immunohistochemistry, and immunoprecipitation assays to assess DNA damage, replication stress, or the status of tumor-associated signaling pathways.
Picture
Picture
Western Blot
WB result of Phospho-Cdc25A (Ser124) Recombinant Rabbit mAb
Primary antibody: Phospho-Cdc25A (Ser124) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg (phosphatase-treated membrane)
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 54 kDa
WB result of Phospho-Cdc25A (Ser124) Recombinant Rabbit mAb
Primary antibody: Phospho-Cdc25A (Ser124) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg (phosphatase-treated membrane)
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 54 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-Phospho-Cdc25A (Ser124) antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Phospho-Cdc25A (Ser124) antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
