WB result of Phospho-c-Kit (Tyr721) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-c-Kit (Tyr721) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NCI-H562 whole cell lysate 20 µg
Lane 2: NCI-H562 starved overnight, then treated with 100 ng/ml hSCF for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 110 kDa
Observed MW: 150 kDa
Phospho-c-Kit (Tyr721) Recombinant Rabbit mAb (S-3481-5)
Phospho-c-Kit (Tyr721) Recombinant Rabbit mAb (S-3481-5)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-c-Kit (Tyr721) |
| Synonyms | Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein (PBT); Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117; KIT |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Cytoplasm |
| Accession | P10721 |
| Clone Number | S-3481-5 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | NCI-H562 (starved overnight, then treated with 100 ng/ml hSCF for 5 minutes) |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Phospho-c-Kit (Tyr721) refers to the phosphorylated form of the c-Kit receptor tyrosine kinase at tyrosine residue 721 within its intracellular juxtamembrane domain. Upon binding of its ligand stem cell factor (SCF), c-Kit undergoes autophosphorylation, including at Tyr721, which serves as a critical docking site for the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K). This phosphorylation event initiates downstream PI3K/Akt signaling cascades, thereby regulating essential cellular processes such as survival, proliferation, differentiation, and migration in hematopoietic stem cells, melanocytes, mast cells, and germ cells. Aberrant phosphorylation at this site has been implicated in various pathological conditions, including certain gastrointestinal stromal tumors (GISTs) and myeloproliferative disorders.
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Western Blot
