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Phospho-ATM (Ser1981) Recombinant Rabbit mAb (S-3396)

Phospho-ATM (Ser1981) Recombinant Rabbit mAb (S-3396)

Catalog Number: S0B6714 Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen ATM
Synonyms Serine-protein kinase ATM; Ataxia telangiectasia mutated (A-T mutated)
Location Nucleus
Accession Q13315
Clone Number S-3396
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample Neocarzinostatin treated HCT 116
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:500-1:1000 Hu
IHC-P 1:250 Hu

Background

Phospho-ATM (Ser1981) refers to the specific activated form of the Ataxia-Telangiectasia Mutated protein, phosphorylated at its serine 1981 residue. ATM is a key PI3K-related kinase that plays a central role in cellular signaling in response to DNA double-strand break damage. The Ser1981 site is located within the kinase domain of ATM. Its autophosphorylation is a hallmark early molecular event for ATM activation following DNA damage: when DNA double-strand breaks occur, ATM rapidly undergoes dimer dissociation and autophosphorylates the Ser1981 site, converting it into an active monomeric form. This phosphorylation event is crucial for the full activation of ATM kinase activity, enabling it to phosphorylate a series of critical downstream substrates (such as p53, CHK2, H2AX, etc.), thereby initiating key cellular responses including cell cycle checkpoint arrest, DNA repair, or apoptosis. Consequently, detecting Phospho-ATM (Ser1981) levels is widely used as a reliable biomarker for DNA damage response activation, holding significant application value in research areas such as tumor radiotherapy, genome stability assessment, and studies related to diseases like Ataxia-Telangiectasia.

Picture

Western Blot

WB result of Phospho-ATM (S1981) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-ATM (S1981) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HCT 116 whole cell lysate 20 µg
Lane 2: HCT 116 treated with 10 µM Neocarzinostatin for 1 hour whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 350 kDa
Observed MW: 350 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti- Phospho-ATM (S1981) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Phospho-ATM (S1981) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Phospho-ATM (S1981) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.