WB result of Phospho-Akt Substrate (RXXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Akt Substrate (RXXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
Phospho-Akt Substrate (RXXST) Recombinant Rabbit mAb (S-2669-104)
Phospho-Akt Substrate (RXXST) Recombinant Rabbit mAb (S-2669-104)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Akt Substrate (RXXST) |
| Immunogen | Synthetic Peptide |
| Clone Number | S-2669-104 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
The Phospho-Akt Substrate (RXXS*/T*) antibody is a specific antibody primarily used to identify and detect substrate proteins phosphorylated by Akt kinase and other related AGC kinase family members. This antibody recognizes protein sequences containing phosphorylated serine or threonine (S*/T*), with the key feature being the presence of an arginine (R) at the -3 position, forming the characteristic R-X-X-pS/pT motif. Within cellular signal transduction, Akt kinase is the central component of the PI3K/Akt pathway, which regulates critical biological processes such as cell growth, survival, proliferation, and metabolism. By targeting this specific phosphorylation motif, the antibody can be employed in various experimental techniques like Western Blot, immunoprecipitation, and immunohistochemistry. This enables researchers to systematically study Akt signaling pathway activity, discover novel downstream substrates, and investigate its role in diseases such as cancer and diabetes.
Picture
Picture
Western Blot
WB result of Phospho-Akt Substrate (RXXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Akt Substrate (RXXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
ELISA
ELISA binding assay shows Phospho-Akt Substrate (RXXST) antibody specifically binds to phospho and non-phospho mitif peptides.
