WB result of Phospho-Akt Substrate (RXRXXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Akt Substrate (RXRXXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
Phospho-Akt Substrate (RXRXXST) Recombinant Rabbit mAb (S-2624-20)
Phospho-Akt Substrate (RXRXXST) Recombinant Rabbit mAb (S-2624-20)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Akt Substrate (RXRXXST) |
| Immunogen | Synthetic Peptide |
| Clone Number | S-2624-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA, WB |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
The Phospho-Akt Substrate (RXRXXS/T) motif is the core sequence specifically recognized and phosphorylated by protein kinase B (Akt). In this motif, R represents arginine, X represents any amino acid, and S/T represents the phosphorylation site serine or threonine. The characteristic arginine residues (RXR) are responsible for specific binding to the catalytic domain of Akt, while the C-terminal serine or threonine (S/T) is the site where Akt catalyzes the phosphorylation reaction. This highly conserved motif is widely found in various downstream substrate proteins of Akt, such as GSK-3β and FOXO transcription factors, and serves as a central hub regulating key signaling pathways involved in cell survival, proliferation, and metabolism. Detecting the phosphorylation level of this motif directly reflects the in vivo activity status of Akt kinase.
Picture
Picture
Western Blot
WB result of Phospho-Akt Substrate (RXRXXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Akt Substrate (RXRXXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
ELISA
ELISA binding assay shows Phospho-Akt Substrate (RXRXXST) Antibody specifically binds to phospho and non-phospho motif peptides.
