WB result of Phospho-Akt (Ser473) Mouse mAb
Primary antibody: Phospho-Akt (Ser473) Mouse mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
Phospho-Akt (Ser473) Mouse mAb (S-769-59)
Phospho-Akt (Ser473) Mouse mAb (S-769-59)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Phospho-Akt (Ser473) |
| Synonyms | RAC-alpha serine/threonine-protein kinase, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, RAC-PK-alpha, PKB, RAC, AKT1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P31749 |
| Clone Number | S-769-59 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB |
| Reactivity | Hu, Rt |
| Predicted Reactivity | Ms |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 |
Background
Akt or Protein kinase B, is a serine/threonine kinase that plays an important role in regulating a number of cellular processes such as growth, metabolism and survival. The importance of the Akt pathway is highlighted by the mutation of various components of the pathway in human cancers such as the PTEN and PI3-kinase (P110α), which occur in more than 30% of human tumors. For Akt to achieve full activation, phosphorylation is needed at both serine 473 (ser473) of the hydrophobic tail and threonine 308 (thr308) of the activation motif, upon growth factor ligation to the receptor tyrosine kinases.
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Western Blot
WB result of Phospho-Akt (Ser473) Mouse mAb
Primary antibody: Phospho-Akt (Ser473) Mouse mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
Dot Blot
Dot blot result of Phospho-Akt (Ser473) Mouse mAb
Lane 1: Phospho-Akt (Ser473) peptide
Lane 2: Akt unmodified peptide
Primary antibody: Phospho-Akt (Ser473) Mouse mAb at 1/1000 dilution
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
