WB result of Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 treated with 0.5 µM oligomycin for 30 minutes whole cell lysate 20 µg
Lane 2: 293T treated with 0.5 µM oligomycin for 30 minutes whole cell lysate 20 µg
Lane 3: MCF7 treated with 0.5 µM oligomycin for 30 minutes whole cell lysate 20 µg (phosphatase treated membrane)
Lane 4: 293T treated with 0.5 µM oligomycin for 30 minutes whole cell lysate 20 µg (phosphatase treated membrane)
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 265 kDa
Observed MW: 270 kDa
This blot was developed with high sensitivity substrate
Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb (S-4302)
Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb (S-4302)
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Acetyl-CoA Carboxylase (Ser79) |
| Synonyms | Acetyl-CoA carboxylase 1; ACC1; Acetyl-Coenzyme A carboxylase alpha (ACC-alpha); ACAC; ACCA; ACACA |
| Location | Cytoplasm |
| Accession | Q13085 |
| Clone Number | S-4302 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | MCF7 treated with 0.5 µM oligomycin for 30 minutes, 293T treated with 0.5 µM oligomycin for 30 minutes |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:250-1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Background
Phospho-Acetyl-CoA Carboxylase (Ser79) refers to the specific phosphorylated state of Acetyl-CoA Carboxylase 1 (ACC1) at serine residue 79, a critical post-translational modification that serves as a key regulatory switch in lipid metabolism. This phosphorylation is primarily catalyzed by AMP-activated protein kinase (AMPK) in response to cellular energy stress or low ATP levels, leading to the allosteric inhibition of ACC enzymatic activity. By inhibiting ACC, the conversion of acetyl-CoA to malonyl-CoA is blocked, which subsequently reduces de novo fatty acid synthesis and relieves the inhibition of carnitine palmitoyltransferase 1 (CPT1), thereby promoting mitochondrial fatty acid oxidation. Consequently, the detection of ACC phosphorylated at Ser79 is widely used as a biomarker for AMPK activation and the metabolic shift from anabolic lipid storage to catabolic energy production, playing a significant role in the physiological responses to exercise, fasting, and pharmacological interventions targeting metabolic disorders.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Phospho-Acetyl-CoA Carboxylase (Ser79) antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in λ-PPase treated paraffin-embedded human colon cancer. Anti- Phospho-Acetyl-CoA Carboxylase (Ser79) antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
