WB result of PHGDH Rabbit pAb
Primary antibody: PHGDH Rabbit pAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 57 kDa
PHGDH Rabbit Polyclonal Antibody
PHGDH Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PHGDH |
| Synonyms | D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; PGDH3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | O43175 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, MCF7, NIH/3T3, mouse brain, C6 |
| Predicted Reactivity | Bv, CyMk, Cz, Or, Pg |
| Purification | Immunogen Affinity |
| Concentration | Lot specific* (generally 0.5 to 2 mg/ml) |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
PHGDH (Phosphoglycerate Dehydrogenase) protein is a key enzyme involved in the metabolic pathway that converts 3-phosphoglycerate to phosphohydroxypyruvate. It plays a crucial role in serine biosynthesis, which is essential for various cellular processes including the production of nucleotides, lipids, and antioxidants. Abnormal expression of PHGDH has been implicated in several diseases, particularly in cancer, where its upregulation can promote tumor growth and survival by supporting biosynthetic demands and redox homeostasis. Additionally, PHGDH has been identified as a potential therapeutic target in certain cancer types due to its role in maintaining cancer cell metabolism.
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Western Blot
WB result of PHGDH Rabbit pAb
Primary antibody: PHGDH Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 57 kDa
WB result of PHGDH Rabbit pAb
Primary antibody: PHGDH Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 57 kDa
