WB result of PGK1 Mouse mAb
Primary antibody: PGK1 Mouse mAb at 1/20000 dilution
Primary antibody incubation conditions: room temperature for 1.5 hours
Lane 1: A-431 whole cell lysate 20 µg
Lane 2: NCCIT whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 40 kDa
PGK1 Mouse mAb
PGK1 Mouse mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | PGK1 |
| Synonyms | Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2 (PRP 2); PGKA |
| Location | Cytoplasm, Mitochondrion |
| Accession | P00558 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein G |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:800 | Hu |
| IF | 1:800 | Ms, Rt |
Background
Phosphoglycerate kinase 1 (PGK1) is a ubiquitous, X chromosome-encoded cytosolic enzyme that catalyzes the reversible transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADP, producing ATP and 3-phosphoglycerate in the payoff phase of glycolysis, thereby serving as one of only two substrate-level phosphorylation steps that directly generate ATP in this pathway. Beyond its canonical metabolic role, PGK1 exhibits significant moonlighting functions: it can translocate to mitochondria under hypoxic or oncogenic signaling to phosphorylate pyruvate dehydrogenase kinase 1 (PDHK1), suppressing pyruvate oxidation and promoting the Warburg effect; it also acts as a protein chaperone facilitating autophagy initiation through interactions with Beclin-1 and ATG proteins, and participates in DNA damage repair and transcriptional regulation via nuclear localization. Clinically, germline loss-of-function mutations in PGK1 cause an X-linked recessive disorder characterized by variable combinations of chronic nonspherocytic hemolytic anemia, myopathy, rhabdomyolysis, neurodevelopmental delay, and seizures due to impaired ATP production in erythrocytes and muscle, while somatic overexpression or activating post-translational modifications are frequently observed in diverse cancers where they drive aerobic glycolysis, chemoresistance, metastasis, and poor prognosis, making PGK1 both a biomarker and a potential therapeutic target for metabolic intervention in malignancy and inherited metabolic disease.
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Western Blot
WB result of PGK1 Mouse mAb
Primary antibody: PGK1 Mouse mAb at 1/20000 dilution
Primary antibody incubation conditions: room temperature for 1.5 hours
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 42 kDa
WB result of PGK1 Mouse mAb
Primary antibody: PGK1 Mouse mAb at 1/20000 dilution
Primary antibody incubation conditions: room temperature for 1.5 hours
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 42 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-PGK1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-PGK1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-PGK1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-PGK1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-PGK1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A431 cells. Anti-PGK1 antibody was used at 1/800 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse testis. Anti-PGK1 antibody was used at 1/800 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat testis. Anti-PGK1 antibody was used at 1/800 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
