PGAM1 Recombinant Rabbit mAb

Catalog Number: S0B60799 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	PGAM1
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	P18669
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, 293T, Jurkat, Hep G2, Raji, HT-1080, RPMI 8226, NIH/3T3, C2C12, mouse liver, mouse brain, mouse kidney, C6, rat liver, rat brain, rat kidney, COS-7
Purification	Protein A
Concentration	1 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:6000	Hu, Ms, Rt, Mk
IHC-P	1:500-1:1000	Hu, Ms, Rt

Background

PGAM1 (phosphoglycerate mutase 1) is a glycolytic enzyme of 254 amino acids (molecular weight ~28.8 kDa) encoded by the PGAM1 gene in the q24.1 region of human chromosome 10, existing as a homodimer, with the active-site His11 residue serving as both donor and acceptor of the phosphate group and catalyzing, with the aid of 2,3-bisphosphoglycerate, the reversible interconversion of 3-phosphoglycerate (3-PG) and 2-phosphoglycerate (2-PG). Its classical function is to maintain glycolytic flux, but more importantly, PGAM1 coordinates glycolysis with anabolism by controlling the intracellular levels of 3-PG and 2-PG: 3-PG acts as a competitive inhibitor of 6-phosphogluconate dehydrogenase in the oxidative pentose phosphate pathway, whereas 2-PG activates phosphoglycerate dehydrogenase in the serine synthesis pathway, thereby channeling glycolytic intermediates toward biosynthesis. PGAM1 is widely overexpressed in various tumors (partly attributable to loss of TP53 function) and promotes tumor progression through both its classical enzymatic activity and non-enzymatic protein–protein interactions (e.g., binding α-smooth muscle actin in breast cancer to regulate the cytoskeleton and migration), making it a promising anticancer target.

Picture

Western Blot

WB result of PGAM1 Recombinant Rabbit mAb
Primary antibody: PGAM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 25 μg
Lane 2: 293T whole cell lysate 25 μg
Lane 3: Jurkat whole cell lysate 25 μg
Lane 4: Hep G2 whole cell lysate 25 μg
Lane 5: Raji whole cell lysate 25 μg
Lane 6: HT-1080 whole cell lysate 25 μg
Lane 7: RPMI 8226 whole cell lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 26 kDa

WB result of PGAM1 Recombinant Rabbit mAb
Primary antibody: PGAM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 25 μg
Lane 2: C2C12 whole cell lysate 25 μg
Lane 3: mouse liver lysate 25 μg
Lane 4: mouse brain lysate 25 μg
Lane 5: mouse kidney lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 26 kDa

WB result of PGAM1 Recombinant Rabbit mAb
Primary antibody: PGAM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 25 μg
Lane 2: rat liver lysate 25 μg
Lane 3: rat brain lysate 25 μg
Lane 4: rat kidney lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 26 kDa

WB result of PGAM1 Recombinant Rabbit mAb
Primary antibody: PGAM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 26 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human brain. Anti-PGAM1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PGAM1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-PGAM1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-PGAM1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-PGAM1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.