WB result of PFKFB4 Recombinant Rabbit mAb
Primary antibody: PFKFB4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
This blot was developed with high sensitivity substrate
PFKFB4 Recombinant Rabbit mAb (S-4762-4)
PFKFB4 Recombinant Rabbit mAb (S-4762-4)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PFKFB4 |
| Synonyms | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; 6PF-2-K/Fru-2,6-P2ase 4; PFK/FBPase 4Alternative name6PF-2-K/Fru-2,6-P2ase testis-type isozyme |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q16877 |
| Clone Number | S-4762-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | Raji, PC-3, U-87 MG |
| Predicted Reactivity | CyMk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
Background
PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4) is a bifunctional metabolic enzyme in the PFKFB family that critically regulates glycolytic flux by catalyzing the synthesis and hydrolysis of fructose-2,6-bisphosphate (F2,6BP). The human PFKFB4 gene is located at chromosome 3p21.31, and its recombinant protein has kinase activity approximately 4 to 4.6 times higher than its phosphatase activity, with its main function favoring F2,6BP synthesis to allosterically activate PFK-1 and promote glycolysis. In normal tissues, PFKFB4 expression is most prominent in the testis, but it is significantly upregulated in various malignant tumors and is induced by hypoxia, being essential for cancer cells to maintain ATP supply and survival under hypoxic conditions. Beyond its metabolic function, PFKFB4 also exhibits "protein kinase" activity, phosphorylating serine 857 of the transcriptional coactivator SRC-3, enhancing its interaction with ATF4 and thereby driving transcription of genes involved in the pentose phosphate pathway and purine synthesis, promoting breast cancer growth and lung metastasis. In glioblastoma, PFKFB4 instead drives tumor progression by activating the AKT/FOXO1 pathway or regulating HIF-1α signaling. Notably, the enzymatic activity bias of PFKFB4 differs across tumor contexts: in hepatocellular carcinoma, its function relies more on phosphatase activity to alleviate ROS stress and mediate sorafenib resistance. Based on its dual roles in tumor metabolic reprogramming and transcriptional regulation, PFKFB4 is regarded as a promising anticancer drug target.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-PFKFB4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PFKFB4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PFKFB4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PFKFB4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
