WB result of Peroxiredoxin 6 Rabbit pAb
Primary antibody: Peroxiredoxin 6 Rabbit pAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 28 kDa
Peroxiredoxin 6 Rabbit Polyclonal Antibody
Peroxiredoxin 6 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Peroxiredoxin 6 |
| Synonyms | 1-Cys peroxiredoxin (1-Cys PRX); 24 kDa protein; Acidic calcium-independent phospholipase A2 (aiPLA2); Antioxidant protein 2; Glutathione-dependent peroxiredoxin; Liver 2D page spot 40; Lysophosphatidylcholine acyltransferase 5 (LPC acyltransferase 5; LPCAT-5; Lyso-PC acyltransferase 5); Non-selenium glutathione peroxidase (NSGPx); Red blood cells page spot 12; PRDX6; AOP2; KIAA0106 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Lysosome |
| Accession | P30041 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, HeLa, HEK-293, MCF7, PC-3, mouse liver, mouse testis, rat liver, rat testis |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Peroxiredoxin-6 (Prdx6) is a unique member of the peroxiredoxin family of antioxidant enzymes. Unlike other peroxiredoxins, Prdx6 contains only one conserved cysteine residue and exhibits three types of activities: peroxidase activity, phospholipase A2 (PLA2) activity, and lysolecithin acyltransferase (LPCAT) activity. These activities are crucial for antioxidant defense, cellular phospholipid balance, and the generation of superoxide anion via activation of NADPH oxidase signaling cascades. The regulation of Prdx6 activities is influenced by subcellular localization, substrate binding, and post-translational modifications. Prdx6 plays a significant role in the reduction of peroxidized membrane phospholipids and in the phospholipid homeostasis by generating lysophospholipid substrates for the remodeling pathway of phospholipid synthesis. Prdx6 is closely associated with the pathogenesis of various diseases, including cancer, inflammatory diseases, ischemic stroke, traumatic brain injury, and neurodegenerative diseases. Moreover, Prdx6 has been implicated in the repair of peroxidized cell membranes and cell signaling, highlighting its importance in maintaining cellular integrity and function. The enzyme's ability to reduce oxidized sn-2 fatty acyl groups of phospholipids and to hydrolyze the sn-2 ester bond of phospholipids contributes to its bifunctional role in antioxidant defense and phospholipid homeostasis.
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Western Blot
WB result of Peroxiredoxin 6 Rabbit pAb
Primary antibody: Peroxiredoxin 6 Rabbit pAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 27 kDa
WB result of Peroxiredoxin 6 Rabbit pAb
Primary antibody: Peroxiredoxin 6 Rabbit pAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 26 kDa
IP
Peroxiredoxin 6 Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating Peroxiredoxin 6 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Peroxiredoxin 6 Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: Peroxiredoxin 6 Rabbit pAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 25 kDa
Observed MW: 26 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Peroxiredoxin 6 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
