WB result of Peroxiredoxin 3 Rabbit pAb
Primary antibody: Peroxiredoxin 3 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 28 kDa
Observed MW: 22~25 kDa
Peroxiredoxin 3 Rabbit Polyclonal Antibody
Peroxiredoxin 3 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Peroxiredoxin 3 |
| Synonyms | Thioredoxin-dependent peroxide reductase, mitochondrial; Antioxidant protein 1 (AOP-1); HBC189; Peroxiredoxin III (Prx-III); Protein MER5 homolog; Thioredoxin-dependent peroxiredoxin 3; PRDX3; AOP1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Mitochondrion |
| Accession | P30048 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, MCF7, A431, mouse heart, rat liver |
| Predicted Reactivity | Bv, Or |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Peroxiredoxin 3 (PRDX3) is a mitochondrial antioxidant enzyme that plays a significant role in maintaining cellular redox balance and protecting cells from oxidative stress. PRDX3 is known to be upregulated during tumorigenesis and cancer progression. It regulates cellular signaling pathways associated with Matrix Metalloproteinase-1 (MMP-1) expression and activity in breast cancer progression. Silencing PRDX3 inhibits cell migration and invasion in triple-negative breast cancer cell lines, while overexpression promotes these processes, which are important in the metastatic cascade. PRDX3 is crucial in the regulation of mitochondrial homeostasis and macrophage polarization. A deficiency of PRDX3 leads to an increase in mitochondrial superoxide levels and the induction of inflammation. PRDX3 is also a mitochondrial hydrogen peroxide scavenger that protects cancer cells against hypoxia and drug-induced hydrogen peroxide-dependent apoptosis. In addition, PRDX3 plays a protective role against mitochondrial dysfunction by removing mitochondrial reactive oxygen species.
Picture
Picture
Western Blot
WB result of Peroxiredoxin 3 Rabbit pAb
Primary antibody: Peroxiredoxin 3 Rabbit pAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 28 kDa
Observed MW: 22~25 kDa
WB result of Peroxiredoxin 3 Rabbit pAb
Primary antibody: Peroxiredoxin 3 Rabbit pAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 28 kDa
Observed MW: 23 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
