IHC shows positive staining in paraffin-embedded human liver. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Peroxiredoxin 1/PAG Recombinant Rabbit mAb (S-1673-22)
Peroxiredoxin 1/PAG Recombinant Rabbit mAb (S-1673-22)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Peroxiredoxin 1/PAG |
| Synonyms | Natural killer cell-enhancing factor A (NKEF-A); Proliferation-associated gene protein (PAG); Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; Thioredoxin-dependent peroxiredoxin 1; PRDX1; PAGA; PAGB; TDPX2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Melanosome |
| Accession | Q06830 |
| Clone Number | S-1673-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, HepG2, A431, A549, MCF7, K562, NIH/3T3, C2C12, mouse kidney |
| Predicted Reactivity | Bv, Hm |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:10000 | Hu, Ms |
| IHC-P | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
Peroxiredoxin 1 (PRDX1) is a member of the peroxiredoxin family of antioxidant enzymes, which are ubiquitously distributed in all species and capable of reducing a broad range of peroxide substrates, including hydrogen peroxide (H2O2) and alkyl hydroperoxides. PRDX1 plays a crucial role in cellular redox homeostasis and is involved in the regulation of various cellular processes such as proliferation, differentiation, and apoptosis. PRDX1 has been implicated in the development and progression of various human cancers, including breast, esophageal, lung, and prostate cancers, where it regulates ROS-dependent signaling pathways and is thought to be a key intracellular intermediate balancing cell survival and apoptosis.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Peroxiredoxin 1/PAG antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
