WB result of PERK Recombinant Rabbit mAb
Primary antibody: PERK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: RD whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 125 kDa
Observed MW: 140 kDa
This blot was developed with high sensitivity substrate
PERK Recombinant Rabbit mAb (S-2822-52)
PERK Recombinant Rabbit mAb (S-2822-52)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PERK |
| Synonyms | Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase (HsPEK); Protein tyrosine kinase EIF2AK3; PEK; EIF2AK3 |
| Immunogen | Recombinant Protein |
| Location | Endoplasmic reticulum |
| Accession | Q9NZJ5 |
| Clone Number | S-2822-52 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Positive Sample | MCF7, 293T, RD, K562, RAW264.7, NIH/3T3 |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
PERK (protein kinase RNA-like endoplasmic reticulum kinase) is an ER-resident transmembrane serine/threonine kinase that, under ER stress caused by misfolded protein accumulation, oligomerizes and auto-phosphorylates to phosphorylate eIF2α, transiently attenuating global cap-dependent translation while selectively up-regulating ATF4 and other stress-response mRNAs, thereby activating a transcriptional program that enhances folding capacity, antioxidant responses, and autophagy, and if stress is unresolved triggers apoptosis, making PERK a central regulator of the unfolded protein response, cancer cell survival, metabolic homeostasis, and neurodegenerative diseases.
Picture
Picture
Western Blot
WB result of PERK Recombinant Rabbit mAb
Primary antibody: PERK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 125 kDa
Observed MW: 140 kDa
This blot was developed with high sensitivity substrate
