WB result of Pericentrin Recombinant Rabbit mAb
Primary antibody: Pericentrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 378 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
Pericentrin Recombinant Rabbit mAb (S-3515-69)
Pericentrin Recombinant Rabbit mAb (S-3515-69)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Pericentrin |
| Synonyms | Kendrin; Pericentrin-B; KIAA0402; PCNT2; PCNT |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cytoskeleton, Centrosome |
| Accession | O95613 |
| Clone Number | S-3515-69 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK293, HepG2, HeLa, MCF7, NIH/3T3, mouse testis, C6, rat testis, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, MK |
| ICC | 1:1000-1:2000 | Hu |
Background
Pericentrin (PCNT, also called kendrin or pericentrin-B) is a large, highly conserved coiled-coil scaffold protein encoded by the PCNT gene on chromosome 21, which localizes to the centrosome and pericentriolar material (PCM) via its C-terminal PACT domain, anchoring γ-tubulin complexes and other regulatory proteins to organize microtubule nucleation and anchoring, thereby orchestrating centrosome assembly, mitotic spindle formation, cell-cycle progression and primary cilium biogenesis; mutations or dysregulation of PCNT underlie disorders such as microcephalic osteodysplastic primordial dwarfism type II, Seckel syndrome, ciliopathies, and have been linked to cancer, mental disorders and altered insulin secretion in β-cells.
Picture
Picture
Western Blot
WB result of Pericentrin Recombinant Rabbit mAb
Primary antibody: Pericentrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 378 kDa
Observed MW: 180 kDa
WB result of Pericentrin Recombinant Rabbit mAb
Primary antibody: Pericentrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 378 kDa
Observed MW: 180 kDa
WB result of Pericentrin Recombinant Rabbit mAb
Primary antibody: Pericentrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 378 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-Pericentrin antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
