Flow cytometric analysis of CD11b expression on human peripheral blood cells. Human peripheral blood cells were stained with PerCP-Cy5.5 Rat IgG2b, κ Isotype Control (left panel) or SDT PerCP-Cy5.5 Rat Anti-Mouse/Human CD11b Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PerCP-Cy5.5 Rat Anti-Mouse/Human CD11b Antibody (M1/70)
PerCP-Cy5.5 Rat Anti-Mouse/Human CD11b Antibody (M1/70)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD11b |
| Location | Cell membrane |
| Accession | P05555、P11215 |
| Clone Number | M1/70 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu, Ms |
| Positive Sample | human peripheral blood cells |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu, Ms |
Background
CD11b, also known as integrin alpha M or CR3A, is a key component of the integrin family, primarily expressed on the surface of immune cells such as macrophages, monocytes, neutrophils, and natural killer cells. It forms a heterodimer with CD18 (integrin β2) to function as a receptor for various molecules, including the iC3b fragment of complement protein, fibrinogen, factor X, and ICAM-1. This receptor complex plays a crucial role in mediating cell adhesion, migration, and the uptake of pathogens and complement-coated particles. Additionally, CD11b is widely used as a marker for identifying macrophages and other immune cells in research. Its involvement in immune responses and cell signaling makes it an important target in studies related to inflammation, immune regulation, and certain diseases.
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FC
Flow cytometric analysis of CD11b expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow were stained with Pacific Blue™ Rat Anti-Mouse Ly6G/Ly6C Antibody and either Rat IgG2b, κ Isotype Control (left panel) or SDT PerCP-Cy5.5 Rat Anti-Mouse/Human CD11b Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
