Flow cytometric analysis of Mouse CD9 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Pacific Blue™ Rat Anti-Mouse Ly-6G/Ly-6C Antibody and either PerCP-Cy5.5 Rat Ig2a, κ Isotype Control (left panel) or SDT PerCP-Cy5.5 Rat Anti-Mouse CD9 Antibody (right panel) at 5μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
PerCP-Cy5.5 Rat Anti-Mouse CD9 Antibody (S-R652)
PerCP-Cy5.5 Rat Anti-Mouse CD9 Antibody (S-R652)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD9 |
| Synonyms | CD9 antigen; Cd9 |
| Location | Secreted, Cell membrane |
| Accession | P40240 |
| Clone Number | S-R652 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD9 is a 24-kDa tetraspanin membrane glycoprotein with four transmembrane domains and two extracellular loops that is widely expressed on hematopoietic and non-hematopoietic cells, where it organizes tetraspanin-enriched microdomains that integrate adhesion, signaling and trafficking by associating with integrins, EWI-2/F, and other tetraspanins to regulate cell adhesion, migration, fusion, exosome biogenesis/secretion, immune activation and tumor suppression, and its large extracellular loop is essential for sperm–egg fusion, while its presence in exosomes marks these vesicles for intercellular communication and its altered expression influences cancer progression, viral entry and stem-cell pluripotency.
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