Flow cytometric analysis of CD172a (SIRPα) expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Brilliant Violet 421™ Rat Anti-Human/Mouse CD11b Antibody and either PerCP-Cy5.5 Rat IgG1, κ Isotype Control (left panel) or SDT PerCP-Cy5.5 Rat Anti-Mouse CD172a (SIRPα) Antibody (right panel) at 5μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
PerCP-Cy5.5 Rat Anti-Mouse CD172a (SIRPα) Antibody (S-R713)
PerCP-Cy5.5 Rat Anti-Mouse CD172a (SIRPα) Antibody (S-R713)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD172a |
| Synonyms | Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs (Bit); CD172 antigen-like family member A; Inhibitory receptor SHPS-1; MyD-1 antigen; Signal-regulatory protein alpha-1 (Sirp-alpha-1; mSIRP-alpha1); p84; Bit; Myd1; Ptpns1; Shps1; Sirp; Sirpa |
| Location | Membrane |
| Accession | P97797 |
| Clone Number | S-R713 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD172a, also known as SIRP alpha (signal-regulatory protein alpha), is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed on cells of myeloid origin, including monocytes, macrophages, dendritic cells, and granulocytes, but not on T and B lymphocytes. SIRP alpha plays a crucial role in immune regulation by interacting with its ligand, CD47, which is widely expressed on various cell types, including cancer cells. This interaction transmits a "do not eat me" signal to prevent phagocytosis of healthy cells and is involved in processes like dendritic cell-mediated T cell activation and neutrophil migration. Additionally, SIRP alpha has regulatory effects on cellular responses induced by growth factors, insulin, and oncogenes, and it is implicated in both physiological and pathological processes, such as cancer progression.
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