THP-1 (Human monocytic leukemia monocyte) was stained with SDT PerCP-Cy5.5 Mouse IgG2b, κ Isotype Control Antibody at 1 μg/test (Red line histogram), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PerCP-Cy5.5 Mouse IgG2b, κ Isotype Control Antibody (S-844-22)
PerCP-Cy5.5 Mouse IgG2b, κ Isotype Control Antibody (S-844-22)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Clone Number | S-844-22 |
| Antibody Type | Mouse mAb |
| Application | FCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Picture
Picture
FC
4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) was stained with SDT PerCP-Cy5.5 Mouse IgG2b, κ Isotype Control Antibody at 1 μg/test (Red line histogram), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
