Human PBMC were stained with Percp-Cy5.5 Mouse IgG2a Isotype Control at 1/20 dilution (1 μg) treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
PerCP-Cy5.5 Mouse IgG2a Isotype Control Antibody (S-R454)
PerCP-Cy5.5 Mouse IgG2a Isotype Control Antibody (S-R454)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | S-R454 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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FC
Flow cytometric analysis of 4% paraformaldehyde fixed and 0.1% Tween permeabilized human PBMC labeled with Percp-Cy5.5 Mouse IgG2a Isotype Control at 1/20 dilution (1 μg) treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
