Flow cytometric analysis of Human CD56 expression on Human PBMC. Human PBMC (peripheral blood mononuclear cells) were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either PerCP-Cy5.5 Mouse IgG1, κ Isotype Control (Left panel) or PerCP-Cy5.5 Mouse Anti-Human CD56 Antibody (Right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PerCP-Cy5.5 Mouse Anti-Human NCAM-1/CD56 Antibody (S-577-58)
PerCP-Cy5.5 Mouse Anti-Human NCAM-1/CD56 Antibody (S-577-58)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | NCAM-1/CD56 |
| Synonyms | Neural cell adhesion molecule 1; N-CAM-1; NCAM; NCAM1 |
| Location | Cell membrane |
| Accession | P13591 |
| Clone Number | S-577-58 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,λ |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
Neural cell adhesion molecule 1 (NCAM-1), also known as CD56, is a cell adhesion protein belonging to the immunoglobulin superfamily. It is expressed on various cell types including neurons, muscle cells, and immune cells like natural killer (NK) cells. NCAM-1/CD56 plays crucial roles in cell-to-cell and cell-matrix interactions during development and differentiation, regulating processes such as neurogenesis, neurite outgrowth, axonal guidance, and cell migration in the nervous system. In the immune system, it is involved in the expansion of T lymphocytes, B lymphocytes, and NK cells. NCAM-1/CD56 also triggers signaling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). Additionally, it serves as a biomarker for certain diseases, such as malignant tumors in the nervous system and neuroendocrine carcinoma.
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