Flow cytometric analysis of Human CD62P expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with APC Anti-Human CD42b Antibody and either APC Mouse IgG1, κ Isotype Control (Left panel) or SDT Percp-Cy5.5 Mouse Anti-Human CD62P (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Percp-Cy5.5 Mouse Anti-Human CD62P Antibody (S-921-3)
Percp-Cy5.5 Mouse Anti-Human CD62P Antibody (S-921-3)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD62P |
| Synonyms | P-selectin; CD62 antigen-like family member P; Granule membrane protein 140 (GMP-140); Leukocyte-endothelial cell adhesion molecule 3 (LECAM3); Platelet activation dependent granule-external membrane protein (PADGEM); GMRP; GRMP; SELP |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P16109 |
| Clone Number | S-921-3 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD62P, also known as P-selectin, is a 140 kDa type I transmembrane glycoprotein that plays a crucial role in cell adhesion and immune responses. It is stored in α-granules of platelets and Weibel-Palade bodies of endothelial cells, and is rapidly relocated to the cell surface upon activation by stimuli such as thrombin or histamine. CD62P mediates the initial adhesion and rolling of leukocytes on endothelial cells during inflammation and facilitates interactions between activated platelets and neutrophils or monocytes. It binds to specific carbohydrate ligands on leukocytes in a calcium-dependent manner. Additionally, CD62P is used as a marker for platelet activation and has potential diagnostic value in various diseases.
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