Flow cytometric analysis of CD4 expression on human peripheral blood leukocytes. Human peripheral blood leukocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 antibody and either PerCP-Cy5.5 Mouse IgG1 Isotype Control (left panel) or SDT PerCP-Cy5.5 Mouse Anti-Human CD4 Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PerCP-Cy5.5 Mouse Anti-Human CD4 Antibody (S-574-16)
PerCP-Cy5.5 Mouse Anti-Human CD4 Antibody (S-574-16)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD4 |
| Synonyms | T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P01730 |
| Clone Number | S-574-16 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Isotype Control | S0B1582 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu |
Background
CD4 (cluster of differentiation 4) is a glycoprotein that serves as a co-receptor for the T-cell receptor (TCR). CD4 is found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells. CD4+ T helper cells are white blood cells that are an essential part of the human immune system. They are often referred to as CD4 cells, T-helper cells or T4 cells. They are called helper cells because one of their main roles is to send signals to other types of immune cells, including CD8 killer cells, which then destroy the infectious particle. If CD4 cells become depleted, for example in untreated HIV infection, or following immune suppression prior to a transplant, the body is left vulnerable to a wide range of infections that it would otherwise have been able to fight.
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