Flow cytometric analysis of CD27 expression on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 and either PerCP-Cy5.5 Mouse IgG1, κ Isotype Control (left panel) or SDT PerCP-Cy5.5 Mouse Anti-Human CD27 Antibody (right panel) at 5 μl/test with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0B88804), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
PerCP-Cy5.5 Mouse Anti-Human CD27 Antibody (S-568-2)
PerCP-Cy5.5 Mouse Anti-Human CD27 Antibody (S-568-2)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD27 |
| Synonyms | CD27 antigen; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7Imported; TNFRSF7 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P26842 |
| Clone Number | S-568-2 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B1582 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood lymphocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily. It is expressed on the surface of various immune cells such as T cells and B cells. CD27 can interact with its ligand CD70, which is mainly found on antigen - presenting cells (APCs) and activated T cells. This interaction plays a crucial role in the regulation of immune responses. It can promote T cell proliferation and differentiation, enhance the cytotoxic activity of T cells against infected or cancerous cells, and also participate in the regulation of B cell functions such as immunoglobulin production. Moreover, the CD27 - CD70 pathway is involved in the development and maintenance of immune memory, helping the immune system to remember and respond more effectively to pathogens it has previously encountered. Abnormal expression or function of CD27 has been implicated in certain immune - related diseases and cancers, making it a potential target for therapeutic interventions in these conditions.
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