Flow cytometric analysis of Human CD127 expression on human peripheral blood leukocytes. Human peripheral blood leukocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 antibody and either PerCP-Cy5.5 Mouse IgG1, κ Isotype Control (Left panel) or SDT PerCP-Cy5.5 Mouse Anti-Human CD127 antibody (Right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PerCP-Cy5.5 Mouse Anti-Human CD127 Antibody (S-R462)
PerCP-Cy5.5 Mouse Anti-Human CD127 Antibody (S-R462)
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Product Details
Product Details
Product Specification
| Synonyms | Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; IL7R |
| Location | Cell membrane |
| Accession | P16871 |
| Clone Number | S-R462 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD127, also known as the interleukin-7 receptor alpha (IL7R-α), is a type I cytokine receptor encoded by the IL7R gene in humans. It is a subunit of the functional interleukin-7 receptor and thymic stromal lymphopoietin (TSLP) receptors. CD127 is expressed on the surface of hematopoietic cells, particularly lymphoid lineage cells, and plays a crucial role in the development, survival, and function of T cells. It is involved in signaling pathways that promote cell survival and proliferation through the activation of JAK1, JAK3, STAT5, and the PI3K-AKT pathway. Additionally, CD127 exists in both membrane-bound and soluble forms, with the soluble form (sCD127) potentially having antagonistic or agonistic effects on IL-7 activity depending on the context.
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