Flow cytometric analysis of Mouse TCR β expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD4 and either PerCP-Cy5.5 Armenian hamster IgG Isotype Control (left panel) or SDT PerCP-Cy5.5 Armenian hamster Anti-Mouse TCR β Antibody (right panel) at 1.25 μl/test treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
PerCP-Cy5.5 Armenian hamster Anti-Mouse TCR β Antibody (H57-597)
PerCP-Cy5.5 Armenian hamster Anti-Mouse TCR β Antibody (H57-597)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | TCR β |
| Synonyms | T-cell receptor beta-1 chain C region |
| Location | Membrane |
| Accession | P01852 |
| Clone Number | H57-597 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 0.25μg per million cells in 100μl volume | Ms |
Background
TCR β (T-cell receptor beta chain) is a crucial component of the T-cell receptor, which, together with the TCR α chain, forms the αβTCR on the surface of T cells. This receptor is responsible for recognizing and binding to antigenic peptide complexes presented by MHC molecules. The diversity of the TCR β chain is generated through a combination of random recombination of multiple germline gene segments, gene rearrangement, junctional diversity, N-region nucleotide insertions, and random pairing of α and β chains. These mechanisms collectively provide the TCR repertoire with immense diversity, enabling T cells to recognize a wide range of antigens.
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