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PE Rat Anti-Mouse Perforin Antibody (S-R644)

PE Rat Anti-Mouse Perforin Antibody (S-R644)

Catalog Number: S0B80531 Application: ICFCM Reactivity: Ms Conjugation: PE Brand: Starter
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Product Details

Product Specification


Host Rat
Antigen Perforin
Synonyms Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; Pfp; Prf1
Location Secreted, Cell membrane
Accession P10820
Clone Number S-R644
Antibody Type Rat mAb
Isotype IgG2a,k
Application ICFCM
Reactivity Ms
Positive Sample C57BL/6 mouse splenocytes
Purification Protein G
Concentration 0.2 mg/ml
Conjugation PE
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.09% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
ICFCM 1μg per million cells in 100μl volume Ms

Background

Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions by forming pores in the membranes of target cells, such as virus-infected or cancerous cells. Once released from immune cells, perforin molecules aggregate and insert into the target cell membrane, creating channels that disrupt the cell's osmotic balance, leading to cell lysis and death. This mechanism is essential for eliminating harmful cells and maintaining the body's overall health.

Picture

FC

Flow cytometric analysis of Mouse Perforin expression in C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then washed and stained in Perm/Wash™ Buffer with APC Rat Anti-Mouse CD49b Antibody and with either PE Rat IgG2a, κ Isotype Control or SDT PE Rat Anti-Mouse Perforin Antibody at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.